SERS spectrum Cytosine

SERS spectrum of Cytosine

A: Raman spectrum from pure material (powder of Cytosine);

B: SERS spectrum (6,25 uM concentration);

C: SERS spectrum (62,5 uM concentration);

D: SERS spectrum (1mM concentration).

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About the analyte

SERS spectra of Cytosine tested in 3 different concentrations and compared to pure material spectra.

SERS substrate used: Randa;

About the analyte: Cytosine is one of the 5 main nitrogenous bases used in storing and transporting genetic information within a cell. It is a pyrimidine derivative, with a heterocyclic aromatic ring and two substituents attached - an amine group at position 4 and a keto group at position 2.

Sampling method: immersion; SERS substrates we immeresed into water solutions of Adenine for ~12 hours and rinsed with deionized water afterwards.

Raman system: Renishaw InVia;

Laser: 785nm, 1mW power;

Lens: 5x magnification;

Integration time: 1 s (measured for 50 s and normalized to 1 s afterwards).